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PEI-folic acid modified carbon nanodots for cancer cell-targeted delivery and two-photon excitation imaging
Wang, Jing; Liu, Jun; wangjing@siom.ac.cn; jliu@siom.ac.cn
2016
发表期刊RSC Adv.
卷号6期号:24页码:19662
摘要Polyethyleneimine conjugated folic acid (PEI-FA) was coated onto the surfaces of fluorescent carbon nanodots (CDots) under neutral conditions through electrostatic interactions between the partially charged amino groups of PEI-FA and the carboxyl (-COOH) groups of the CDot surfaces. Because of the high abundance of folate receptors (FR) in cancer cells, folic acid (FA) was used as the targeting ligand to enhance the CDots' binding capability and penetration into the target cancer cells. We evaluated the amount of particles internalized by cancer cell lines displaying various levels of folate receptors. Two-photon excitation microscopy images revealed that the uptake of CDot-PEI-FA by FR-positive KB cancer cells was much higher than that by FR-negative A549 cancer cells. Moreover, quantitative flow cytometry analysis confirmed the receptor-mediated targeted delivery of the nanoparticles into different FR-expressing cells. The internalization of particles was ten-fold higher in the FR-positive KB cancer cells compared to that in the FR-negative A549 cancer cells; the difference was significant enough to be of biological importance. These preliminary results demonstrate the promising potential of CDot-PEI-FA for FR-positive cancer cell line diagnosis.
文章类型Article
部门归属强光
DOI10.1039/c5ra27249a
资助者National Natural Science Foundation of China (NSFC) [61178006, 11274327, 61221064, 61527821]; Recruitment Program of Global Youth Experts ; National Natural Science Foundation of China (NSFC) [61178006, 11274327, 61221064, 61527821]; Recruitment Program of Global Youth Experts ; National Natural Science Foundation of China (NSFC) [61178006, 11274327, 61221064, 61527821]; Recruitment Program of Global Youth Experts ; National Natural Science Foundation of China (NSFC) [61178006, 11274327, 61221064, 61527821]; Recruitment Program of Global Youth Experts
收录类别SCI
资助者National Natural Science Foundation of China (NSFC) [61178006, 11274327, 61221064, 61527821]; Recruitment Program of Global Youth Experts ; National Natural Science Foundation of China (NSFC) [61178006, 11274327, 61221064, 61527821]; Recruitment Program of Global Youth Experts ; National Natural Science Foundation of China (NSFC) [61178006, 11274327, 61221064, 61527821]; Recruitment Program of Global Youth Experts ; National Natural Science Foundation of China (NSFC) [61178006, 11274327, 61221064, 61527821]; Recruitment Program of Global Youth Experts
WOS记录号WOS:000371122900004
引用统计
文献类型期刊论文
条目标识符http://ir.siom.ac.cn/handle/181231/28684
专题强场激光物理国家重点实验室
通讯作者wangjing@siom.ac.cn; jliu@siom.ac.cn
作者单位中国科学院上海光学精密机械研究所
推荐引用方式
GB/T 7714
Wang, Jing,Liu, Jun,wangjing@siom.ac.cn,et al. PEI-folic acid modified carbon nanodots for cancer cell-targeted delivery and two-photon excitation imaging[J]. RSC Adv.,2016,6(24):19662.
APA Wang, Jing,Liu, Jun,wangjing@siom.ac.cn,&jliu@siom.ac.cn.(2016).PEI-folic acid modified carbon nanodots for cancer cell-targeted delivery and two-photon excitation imaging.RSC Adv.,6(24),19662.
MLA Wang, Jing,et al."PEI-folic acid modified carbon nanodots for cancer cell-targeted delivery and two-photon excitation imaging".RSC Adv. 6.24(2016):19662.
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