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Photostability Enhancement of Fluorenone-Based Two-Photon Fluorescent Probes for Cellular Nucleus Monitoring and Imaging
Chi, Shuheng; Li, Liang; Wu, Yiqun; yqwu@siom.ac.cn
2016
发表期刊J. Phys. Chem. C
卷号120期号:25页码:13706
摘要A series of fluorenone-based two-photon fluorescent probes with high photostability for nucleus imaging are prepared and developed. The one- and two-photon photophysical properties exhibit these new probes possess 0.448-0.634 of fluorescence quantum yields and 469-495 GM of two-photon absorption cross sections at 800 nm femtosecond laser excitation. The luminescence "turn-on" experiment in buffer solutions indicates that 35-fold of fluorescence intensity and 68-fold fluorescence quantum yield enhancement appear between new probes and calf thymus DNA. In the nuclear double staining experiment, the high mean colocalization coefficients of 0.92-0.96 between new probes and nuclear labeling dye Hoechst 33342 are acquired, demonstrating excellent nuclear localization in 3T3 cells. The counterstain studies by introducing commercial mitochondrial staining reagent MTR and nuclear staining dye DAPI further show good membrane permeability and counterstain compatibility in multicolor cell labeling application. The photostability studies show that 3000 s of observation time and 0.028%/s-0.03%/s of mean fluorescence attenuation rates under persistent laser irradiation in two-photon nuclear imaging are achieved. Meanwhile, the cause of fluorescence attenuation in the photostability test for cellular nuclei monitoring are discussed as well.
文章类型Article
部门归属存储
DOI10.1021/acs.jpcc.6b02827
资助者National Natural Science Foundation of China [61137002, 61178059, 51172253] ; National Natural Science Foundation of China [61137002, 61178059, 51172253] ; National Natural Science Foundation of China [61137002, 61178059, 51172253] ; National Natural Science Foundation of China [61137002, 61178059, 51172253]
收录类别SCI
资助者National Natural Science Foundation of China [61137002, 61178059, 51172253] ; National Natural Science Foundation of China [61137002, 61178059, 51172253] ; National Natural Science Foundation of China [61137002, 61178059, 51172253] ; National Natural Science Foundation of China [61137002, 61178059, 51172253]
WOS记录号WOS:000379456800046
引用统计
文献类型期刊论文
条目标识符http://ir.siom.ac.cn/handle/181231/28250
专题高密度光存储技术实验室
通讯作者yqwu@siom.ac.cn
作者单位中国科学院上海光学精密机械研究所
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Chi, Shuheng,Li, Liang,Wu, Yiqun,et al. Photostability Enhancement of Fluorenone-Based Two-Photon Fluorescent Probes for Cellular Nucleus Monitoring and Imaging[J]. J. Phys. Chem. C,2016,120(25):13706.
APA Chi, Shuheng,Li, Liang,Wu, Yiqun,&yqwu@siom.ac.cn.(2016).Photostability Enhancement of Fluorenone-Based Two-Photon Fluorescent Probes for Cellular Nucleus Monitoring and Imaging.J. Phys. Chem. C,120(25),13706.
MLA Chi, Shuheng,et al."Photostability Enhancement of Fluorenone-Based Two-Photon Fluorescent Probes for Cellular Nucleus Monitoring and Imaging".J. Phys. Chem. C 120.25(2016):13706.
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