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Novel mono-cationic fluorescent probes based on different central pi-conjugated bridges for two-photon bioimaging of cellular nuclei
Chi, Shuheng; Li, Liang; Wu, Yiqun; yqwu@siom.ac.cn
2016
发表期刊RSC Adv.
卷号6期号:74页码:69748
摘要A series of novel pyridine mono-cationic two-photon fluorescent probes based on different central pi-conjugated bridges, fluorenone (W-pyI), dibenzothiophene (S-pyI), and dibenzofuran (F-pyI), were prepared and studied. Under one-photon excitation in a N,N-dimethylformamide solution, W-pyI, S-pyI, and F-pyI displayed fluorescence quantum yields of 0.401, 0.425, and 0.09, respectively. The two-photon fluorescence performance indicated that these probes possessed two-photon absorption cross-sections of 681 GM (W-pyI), 630 GM (S-pyI), and 620 GM (F-pyI) at 800 nm femtosecond laser excitation. The luminance "turn-on" effect of W-pyI, S-pyI, and F-pyI bonding with calf thymus DNA in Tris-HCl buffer solutions displayed 58-fold, 30-fold, and 25-fold fluorescence quantum yield increments, respectively, and 350-450% two-photon absorption cross-section enhancement. The confocal fluorescence imaging showed clear one-and two-photon fluorescence imaging. The mean co-localization coefficients between these probes and Hoechst 33342 in 3T3 cells ranged from 0.89-0.92, indicating that they showed excellent nuclear targeting abilities. The counterstaining experiments showed these probes possessed good counterstaining compatibility and membrane permeability in the application of multicolor targeting. A time-dependent fluorescence intensity test under continuous femtosecond laser irradiation showed that W-pyI possessed a longer observation time (3000 seconds) and a lower fluorescence attenuation amplitude (7.1%) in the first 300 seconds than S-pyI, F-pyI and other previously reported pyridinium derivatives, demonstrating that the central pi-conjugated bridge "fluorenone" played a key role in improving photostability during probe designing for two-photon bioimaging applications.
文章类型Article
部门归属存储
DOI10.1039/c6ra12193d
资助者National Natural Science Foundation of China [61137002, 61178059, 51172253] ; National Natural Science Foundation of China [61137002, 61178059, 51172253] ; National Natural Science Foundation of China [61137002, 61178059, 51172253] ; National Natural Science Foundation of China [61137002, 61178059, 51172253]
收录类别SCI
资助者National Natural Science Foundation of China [61137002, 61178059, 51172253] ; National Natural Science Foundation of China [61137002, 61178059, 51172253] ; National Natural Science Foundation of China [61137002, 61178059, 51172253] ; National Natural Science Foundation of China [61137002, 61178059, 51172253]
WOS记录号WOS:000381512800012
引用统计
文献类型期刊论文
条目标识符http://ir.siom.ac.cn/handle/181231/28247
专题高密度光存储技术实验室
通讯作者yqwu@siom.ac.cn
作者单位中国科学院上海光学精密机械研究所
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GB/T 7714
Chi, Shuheng,Li, Liang,Wu, Yiqun,et al. Novel mono-cationic fluorescent probes based on different central pi-conjugated bridges for two-photon bioimaging of cellular nuclei[J]. RSC Adv.,2016,6(74):69748.
APA Chi, Shuheng,Li, Liang,Wu, Yiqun,&yqwu@siom.ac.cn.(2016).Novel mono-cationic fluorescent probes based on different central pi-conjugated bridges for two-photon bioimaging of cellular nuclei.RSC Adv.,6(74),69748.
MLA Chi, Shuheng,et al."Novel mono-cationic fluorescent probes based on different central pi-conjugated bridges for two-photon bioimaging of cellular nuclei".RSC Adv. 6.74(2016):69748.
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