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The conjugates of carbon nanodots and chlorin e6 for enhancing cellular internalization and photodynamic therapy of cancers
Wang, Jing; Wang, Xiongwei; Wang, Shimiao; Huang, Zheng; Liu, Jun; jliu@siom.ac.cn
2016
Source PublicationLaser Phys. Lett.
Volume13Issue:9
AbstractChlorin e6 (Ce6), a large heterocyclic aromatic molecule, is a promising photosensitizer for photodynamic therapy (PDT). We propose an efficient nano-photosensitizer delivery system based on covalent interactions between Ce6 and polyethylenimine (PEI) coated carbon nanodots (CDots). We observed >50% Ce6 drug loading content for PEI, due to this compound's unique 'proton sponge effect.' We found that the covalently incorporated Ce6 molecules retained their functional properties for near-infrared (NIR) fluorescence imaging and PDT. The chemical characteristics of CDot-PEI-Ce6 and Ce6 were evaluated using different analytical methods, including transmission electron microscopy and UV-Visible absorption spectra. Time-correlated single photon counting (TCSPC) and fluorescence spectra were used to demonstrate that Ce6 successfully conjugated to the CDots. The high cellular uptake of CDots-PEI-Ce6 was confirmed using flow cytometry and confocal laser scanning microscopy. According to the MTT assay, the CDots-PEI-Ce6 exhibited low dark toxicity and efficient PDT efficacy to HeLa cancer cells. These results indicate that CDot-PEI-Ce6 conjugates are potential photosensitizer delivery systems for PDT.
SubtypeArticle
Department强光
DOI10.1088/1612-2011/13/9/095602
Funding OrganizationNational Natural Science Foundation of China (NSFC) [11274327, 61521093, 61527821]; Chinese Academy of Sciences [YZ201538] ; National Natural Science Foundation of China (NSFC) [11274327, 61521093, 61527821]; Chinese Academy of Sciences [YZ201538] ; National Natural Science Foundation of China (NSFC) [11274327, 61521093, 61527821]; Chinese Academy of Sciences [YZ201538] ; National Natural Science Foundation of China (NSFC) [11274327, 61521093, 61527821]; Chinese Academy of Sciences [YZ201538]
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China (NSFC) [11274327, 61521093, 61527821]; Chinese Academy of Sciences [YZ201538] ; National Natural Science Foundation of China (NSFC) [11274327, 61521093, 61527821]; Chinese Academy of Sciences [YZ201538] ; National Natural Science Foundation of China (NSFC) [11274327, 61521093, 61527821]; Chinese Academy of Sciences [YZ201538] ; National Natural Science Foundation of China (NSFC) [11274327, 61521093, 61527821]; Chinese Academy of Sciences [YZ201538]
WOS IDWOS:000383980900011
Citation statistics
Document Type期刊论文
Identifierhttp://ir.siom.ac.cn/handle/181231/27743
Collection强场激光物理国家重点实验室
Corresponding Authorjliu@siom.ac.cn
Affiliation中国科学院上海光学精密机械研究所
Recommended Citation
GB/T 7714
Wang, Jing,Wang, Xiongwei,Wang, Shimiao,et al. The conjugates of carbon nanodots and chlorin e6 for enhancing cellular internalization and photodynamic therapy of cancers[J]. Laser Phys. Lett.,2016,13(9).
APA Wang, Jing,Wang, Xiongwei,Wang, Shimiao,Huang, Zheng,Liu, Jun,&jliu@siom.ac.cn.(2016).The conjugates of carbon nanodots and chlorin e6 for enhancing cellular internalization and photodynamic therapy of cancers.Laser Phys. Lett.,13(9).
MLA Wang, Jing,et al."The conjugates of carbon nanodots and chlorin e6 for enhancing cellular internalization and photodynamic therapy of cancers".Laser Phys. Lett. 13.9(2016).
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