SIOM OpenIR  > 强场激光物理国家重点实验室
生物单分子光学探测方法的进展
Alternative TitleDevelopment on Biologic Single Molecular Optical Detection
王桂英; 王琛; 徐至展; 陈宜张
2003
Source Publication激光生物学报
Volume12Issue:3Pages:174
Abstract活细胞中单分子的实时显视是单分子生物学的关键技术,本文针对单分子显视的光学方法做了评述。分别描述了共焦荧光显微术、荧光全内反射显微术以及荧光共振能量转移探测的技术细节,分析了这些技术对于单分子探测所具备的优势和不足。并对单分子方法的未来发展化出预测。指出包括原子力在内的各种探测手段的联合使用和创新荧光染料技术是进一步提高分辨率的突破口。而随着高灵敏和低噪音探测器的发展,各种新方法的出现也有可能突破目前荧光染料尺度给予的分辨极限。
SubtypeReview
Other AbstractDue to biologic single molecular visualization in real time is a key technology to develop molecule biologies, optical detection aperture as a sensitive detection tool is reviewed in this paper. Confocal fluorescent microscopy, fluorescent total internal reflection microscopy and fluorescent resonance energy transfer detection technique are described respectively and analyzed for their advantage and shortcoming in the biologic single molecule detection. The AFM is a fine instrument capable of imaging single macromolecules with submolecular resolution. So AFM combines other optical detection methods will be a powerful tools. Furthermore, innovating technique on fluorochrome is also a rising optical resolution aperture. The resolution limitation given by the fluorochrome may be broken through with new technology, for example, reducing the fluorochrome and developing more sensitive sizeand lower noise detector, etc.
Funding Organization国家自然科学基金(60078025) ; 国家科技部重大基础研究基金的资助(2002CB713808) ; 国家自然科学基金(60078025) ; 国家科技部重大基础研究基金的资助(2002CB713808)
Indexed ByCSCD
Language中文
Funding Organization国家自然科学基金(60078025) ; 国家科技部重大基础研究基金的资助(2002CB713808) ; 国家自然科学基金(60078025) ; 国家科技部重大基础研究基金的资助(2002CB713808)
CSCD IDCSCD:1202013
Citation statistics
Document Type期刊论文
Version出版稿
Identifierhttp://ir.siom.ac.cn/handle/181231/18283
Collection强场激光物理国家重点实验室
Affiliation1.Wang Guiying, Shanghai Institute of Optics and Fine Mechanics, the Chinese Academy of Sciences, Shanghai 201800, China.
2.Wang Chen, Shanghai Institute of Optics and Fine Mechanics, the Chinese Academy of Sciences, Shanghai 201800, China.
3.Xu Zhizhan, Shanghai Institute of Optics and Fine Mechanics, the Chinese Academy of Sciences, Shanghai 201800, China.
4.Chen Yizhang, Medical College of Zhejiang University, Hangzhou, Zhejiang 310031, China.
Recommended Citation
GB/T 7714
王桂英,王琛,徐至展,等. 生物单分子光学探测方法的进展[J]. 激光生物学报,2003,12(3):174.
APA 王桂英,王琛,徐至展,&陈宜张.(2003).生物单分子光学探测方法的进展.激光生物学报,12(3),174.
MLA 王桂英,et al."生物单分子光学探测方法的进展".激光生物学报 12.3(2003):174.
Files in This Item:
File Name/Size DocType Version Access License
297.pdf(162KB)期刊论文作者接受稿开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[王桂英]'s Articles
[王琛]'s Articles
[徐至展]'s Articles
Baidu academic
Similar articles in Baidu academic
[王桂英]'s Articles
[王琛]'s Articles
[徐至展]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[王桂英]'s Articles
[王琛]'s Articles
[徐至展]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.