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全内反射荧光显徽术
Alternative TitleTotal internal reflection fluorescence microscopy
王琛; 王桂英; 徐至展
2002
Source Publication物理学进展
Volume22Issue:4Pages:406
Abstract全内反射荧光显微技术是当今世界上最具前途的新型生物光学显微技术之一,可以用来实现对单个荧光分子的直接深测。它利用全内反射产生的隐失波照明样品,使照明区域限定在样品表面的一薄层范围内,因此具有其它光学成像技术无法比拟的高的信噪比和对比度。近年来,已被生物物理学家们广泛应用于单分子的荧光成像中。本文系统的介绍了全内反射荧光显微技术的原理、国内外的发展和现状及其在生物学上的应用,并对其未来做了展望。
SubtypeArticle
Other AbstractThe total internal reflection fluorescence microscopy is one of the most promising bio-optical techniques in the world, which has been used to observe single fluorescent molecule directly. This specialized technique specifically illuminates sample with evanescent wave created by total internal reflection, and only a few hundred nanometers depth of sample surface should be excited. As a result, it provides the highest signal-to-noise ratio and contrast that is unmatched by any other light microscopy technique. Recently, it is gaining popularity with biophysicist to observe single fluorescent molecules. In this paper, the principle of the total internal reflection fluorescence microscopy and the process at home and abroad are systematically reviewed, as well as the application in cell biology is introduced. Future prospect of this technology are also discussed.
Funding Organization上海市科学技术发展基金(01DJGK018) ; 国家自然科学基金(No. 60078025) ; 国家科技部重大基础研究基金的资助(G1999075200) ; 上海市科学技术发展基金(01DJGK018) ; 国家自然科学基金(No. 60078025) ; 国家科技部重大基础研究基金的资助(G1999075200)
Indexed ByCSCD
Language中文
Funding Organization上海市科学技术发展基金(01DJGK018) ; 国家自然科学基金(No. 60078025) ; 国家科技部重大基础研究基金的资助(G1999075200) ; 上海市科学技术发展基金(01DJGK018) ; 国家自然科学基金(No. 60078025) ; 国家科技部重大基础研究基金的资助(G1999075200)
CSCD IDCSCD:1147742
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Document Type期刊论文
Version出版稿
Identifierhttp://ir.siom.ac.cn/handle/181231/18015
Collection强场激光物理国家重点实验室
Affiliation1.王琛, 中科院上海光学精密机械所, 上海光学精密仪器研究所强光光学开放实验室, 上海 201800, 中国.
2.王桂英, 中科院上海光学精密机械所, 上海光学精密仪器研究所强光光学开放实验室, 上海 201800, 中国.
3.徐至展, 中科院上海光学精密机械所, 上海光学精密仪器研究所强光光学开放实验室, 上海 201800, 中国.
Recommended Citation
GB/T 7714
王琛,王桂英,徐至展. 全内反射荧光显徽术[J]. 物理学进展,2002,22(4):406.
APA 王琛,王桂英,&徐至展.(2002).全内反射荧光显徽术.物理学进展,22(4),406.
MLA 王琛,et al."全内反射荧光显徽术".物理学进展 22.4(2002):406.
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